microarray‐based gene expression analysis guide (v.6.5)  (Agilent technologies)

Journal: Frontiers in Plant Science

Article Title: A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

doi: 10.3389/fpls.2016.01979

Figure Lengend Snippet: Expression analyses in petunia petals by qPCR. (A) Expression analysis of F3 ′ H and N21 in the ph3 mutant and in VvWRKY26 expressing lines as confirmation of the microarray results. (B) Expression analysis of structural genes related to vacuolar acidification (PhPH5 and PhPH1) and to anthocyanin synthesis (PhCHS-A and PhDFR-A) in the untransformed R27 and ph3 lines and VvWRKY26 expressing plants. In all analyses the data correspond to the mean ± SE of three biological replicates (corresponding to lines 1, 2, and 5; Supplementary Figure ) relative to an ACTIN housekeeping control and normalized against the ph3 mutant value. Abbreviations correspond to: PhF3 ′ H, FLAVONOID-3 ′ -MONOOXYGENASE; PhN21, NODULIN MTN21-LIKE PROTEIN; PhPH5, H + P 3 A -ATPASE; PhPH1, P 3 B -ATPASE; PhCHS-A, CHALCONE SYNTHASE A; PhDFR-A, DIHYDROFLAVONOL 4-REDUCTASE A .

Article Snippet: For microarray analysis on Sultana leaves transiently over-expressing VvWRKY26 , the cDNA synthesis, labeling, hybridization and washing reactions were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).

Techniques: Expressing, Mutagenesis, Microarray